The Effects of Menopausal Status, Isoflavone Supplementation and Training on Cardiometabolic Function Women

The goal of this clinical trial is to investigate the role of estrogen and isoflavone supplementation in exercise-induced cellular signaling and cardiometabolic adaptations in premenopausal and postmenopausal women. The study will evaluate vascular function, mitochondrial function, acute and long-term exercise responsiveness, and menopause-related outcomes. The main questions it aims to answer are: * Does menopause alter acute exercise-induced activation of cardiometabolic signaling pathways and vascular function? * Does isoflavone supplementation enhance exercise-induced improvements in vascular and mitochondrial function in postmenopausal women? * Are training-induced adaptations in vascular and mitochondrial function different between premenopausal and postmenopausal women? Researchers will compare postmenopausal women receiving isoflavone supplementation with postmenopausal women receiving placebo to determine the specific effects of isoflavones. Premenopausal women will serve as a reference group to evaluate menopause-related differences. Participants will: * Complete an 8-week supervised exercise training intervention consisting of three training sessions per week. * Undergo assessments of vascular function, including flow-mediated dilation (FMD) and intra-arterial infusions of acetylcholine, epoprostenol, and vitamin C. * Provide blood and skeletal muscle samples for analyses of cellular signaling pathways, protein expression, and mitochondrial function. * Undergo measurements of aerobic capacity, strength and body composition. * Complete questionnaires related to menopause-associated symptoms and health outcomes. Postmenopausal participants will be randomized to receive either isoflavone supplementation or placebo for 4 weeks prior to and throughout the exercise intervention.