This interventional single-arm pre-post study aimed to investigate the acute effects of repeated Wingate exercise on free thyroid hormone responses in male amateur soccer players. Twenty healthy male amateur soccer players aged 19-22 years completed a standardized repeated Wingate exercise protocol. Blood samples were collected immediately before and after exercise to determine serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations. The study evaluated the immediate endocrine responses to repeated high-intensity anaerobic exercise and their potential implications for exercise physiology and thyroid hormone regulation.