This study evaluated whether adding Pilates exercises to a desk-based exercise program provides greater benefits for female office workers than desk-based exercises alone or no exercise intervention. The study included 42 female office workers who were randomly assigned to three groups: a desk-based exercise group, a combined desk-based exercise and Pilates group, or a control group. The exercise interventions were performed for 6 weeks, three days per week. Pain, posture, core muscle endurance, burnout, and hedonic eating behavior were assessed before and after the intervention.