The purpose of this randomized crossover study was to compare the acute effects of repeated-sprint protocols incorporating different numbers of 180-degree changes of direction in adolescent female basketball players. Twenty participants completed three exercise conditions in a randomized, counterbalanced order: 10 × 15-m linear sprints, 10 × 15-m sprints incorporating one 180-degree change of direction, and 10 × 15-m sprints incorporating two 180-degree changes of direction. Each sprint was followed by 30 seconds of passive recovery. Sprint performance, perceived exertion, heart rate, peripheral oxygen saturation, and systolic and diastolic blood pressure were assessed. The study examined whether increasing the number of changes of direction affected repeated-sprint performance and acute perceptual and cardiovascular responses.