Effects of Coca-Cola Zero Sugar Consumption on Urinary Metabolites in Healthy Adults

Consumption of beverages containing non-nutritive sweeteners has increased worldwide, but limited information is available regarding the short-term urinary excretion of acesulfame potassium (Ace-K) and the acute metabolic response following consumption of commercially available beverages. This study investigates the urinary pharmacokinetics of Ace-K and the acute urinary metabolomic response after consumption of 500 mL of Coca-Cola Zero Sugar in healthy adults. Participants complete two study arms under free-living conditions: Coca-Cola Zero Sugar and still water (control), separated by a one-month washout period. Urine samples are collected before beverage consumption and repeatedly over the subsequent 24 hours. Proton nuclear magnetic resonance (¹H NMR) spectroscopy is used to quantify Ace-K and other urinary metabolites. The study aims to determine the urinary appearance and elimination kinetics of Ace-K and to identify metabolic changes associated with Coca-Cola Zero Sugar consumption compared with water.

NCT Number	NCT07694401
Lead Sponsor	Czech University of Life Sciences Prague
Conditions	Healthy Adult Participants
Enrollment	10 participants
Start Date	2023-09-01
Primary Completion	2024-06-15 (estimated)
Study Completion	2024-09-30 (estimated)
Updated on ClinicalTrials.gov	2026-07-23