Bacterial Profile Changes During Different Sodium Hypochlorite Irrigation Regimens and Calcium Hydroxide Medicament in Root Canal Treatment

The goal of this clinical trial is to learn how the bacterial profile in infected root canals changes during different phases of routine root canal treatment in adults with chronic apical abscess. The main questions it aims to answer are: * How does the root canal bacterial profile change before and after irrigation with 5.25 percent sodium hypochlorite or 3 percent sodium hypochlorite? * How does the bacterial profile change after calcium hydroxide medicament is placed in the root canal? Participants will: * Receive standard root canal treatment with one of two irrigation regimens (5.25 percent or 3 percent sodium hypochlorite, plus EDTA), * Have root canal samples taken three times (before any treatment, after irrigation, and after calcium hydroxide medicament), and * Have the bacteria in these samples analyzed using 16S rRNA PCR and DNA sequencing.

NCT Number	NCT07623278
Lead Sponsor	Hasanuddin University
Conditions	Chronic Apical Abscess, Root Canal Infection
Enrollment	24 participants
Start Date	2025-10-13
Primary Completion	2026-03-30 (estimated)
Study Completion	2026-05-18 (estimated)
Updated on ClinicalTrials.gov	2026-06-05