Anterior cruciate ligament (ACL) injuries commonly occurred through non-contact mechanisms during dynamic tasks such as single-leg landing (SLL). Trunk control and lower limb muscle coordination were believed to play a critical role in modulating knee joint biomechanics and ACL loading; however, their individual muscle contributions remained poorly understood due to the difficulty of in-vivo ACL force measurement. This cross-sectional study aimed to investigate the relationship between core strength, lower limb muscle forces, knee joint biomechanics, and ACL loading during single-leg landing in collegiate athletes. Three-dimensional full-body kinematics, ground reaction forces, and electromyography data were collected and integrated into a musculoskeletal modelling framework to estimate ACL loading and individual muscle force contributions. Findings from this study were expected to provide biomechanical evidence to support targeted injury-prevention and rehabilitation strategies.