Radiation therapy is an important treatment for cancer, but patients receiving radiation therapy for the first time may experience anxiety because they are unfamiliar with the treatment process, equipment, and possible side effects. This study evaluated whether a 360-degree virtual reality patient education program could improve radiation therapy knowledge and reduce anxiety and depression among first-time radiation therapy patients. Participants received either virtual reality education or conventional nurse-led education. Knowledge, anxiety, and depression were assessed before and after the educational intervention.