Effects Of Dietary Fats on Gut Microbiota Composition and Metabolic Activity in Healthy Adults

This study will investigate how adding a specific type of dietary fat to daily meals for two weeks affects the gut bacteria and their activity. Participants will be randomly assigned to consume one of four types of fat: butter, coconut oil, olive oil, or sunflower oil (60 mL each day for 2 weeks). Stool and blood samples (after an overnight fast) will be collected at 3 time points during the study (Days 1, 7, and 22). These samples will be used to measure metabolites produced by gut bacteria, the types of bacteria present in the gut, blood lipids, and inflammatory markers. The study, based on previous in vitro findings, aims to understand whether different types of fats (based on their structure and level of saturation) have different effects on gut bacteria and their activity in healthy subjects.

NCT Number	NCT07550023
Lead Sponsor	University of Glasgow Collaborators: Dr Dalia Malkova, Professor Konstantinos Gerasimidis
Conditions	Short Chain Fatty Acids Concentration in Stools, Gut Microbiota Diversity and Composition, Blood Lipid Profiles, Inflammation Biomarkers
Enrollment	64 participants
Start Date	2026-03-23
Primary Completion	2027-03-31 (estimated)
Study Completion	2028-03-31 (estimated)
Updated on ClinicalTrials.gov	2026-04-24