Metagenomic Analysis and Postoperative Pain in Infected Root Canals Using Different Irrigation Protocols

Achieving effective root canal disinfection while minimizing postoperative pain is a critical goal in endodontic therapy. Advanced irrigation protocols, such as ultrasonic activation, laser activation, negative pressure irrigation (PulpSucker device), and mechanical activation (Easy Clean device), have shown promise in enhancing bacterial reduction within the root canal system. However, their impact on postoperative pain has not been fully explored. This randomized controlled clinical trial aims to compare bacterial reduction and postoperative pain levels in infected root canals using four different irrigation protocols. The study will use metagenomic analysis to evaluate changes in bacterial community composition and the Visual Analogue Scale (VAS) to assess patient-reported pain levels at multiple time points postoperatively.

NCT Number	NCT07546773
Lead Sponsor	Suez Canal University
Conditions	Dental Pulp Necrosis, Pulpitis
Enrollment	40 participants
Start Date	2026-04-30
Primary Completion	2027-08-15 (estimated)
Study Completion	2028-01-15 (estimated)
Updated on ClinicalTrials.gov	2026-05-04