Apple Intake Effect on Faecal Metabolome

This pilot dietary intervention study aimed to investigate whether short-term consumption of apples influences the human faecal metabolomic profile. Apples are a commonly consumed source of dietary fibre, particularly pectin, which undergoes microbial fermentation in the colon and may lead to measurable metabolic changes. Healthy adult volunteers were instructed to consume three apples per day for three consecutive days under free-living conditions. Faecal samples were collected before and after the intervention and analysed using proton nuclear magnetic resonance (¹H NMR) spectroscopy to assess changes in metabolite concentrations. The study sought to explore whether metabolomic profiling can detect functional microbial responses to increased fruit intake and provide preliminary insight into the metabolic impact of short-term dietary fibre exposure.

NCT Number	NCT07499960
Lead Sponsor	Czech University of Life Sciences Prague
Conditions	Healthy Adult Participants
Enrollment	15 participants
Start Date	2023-04-01
Primary Completion	2024-08-30 (estimated)
Study Completion	2025-02-25 (estimated)
Updated on ClinicalTrials.gov	2026-04-01