Pesticides and Infertility: Oxidative Stress Via Circulating Cell-free DNA and Gut/Genital Microbiome Signatures in Women With Endometriosis

This project PestiEndoMicro aims to provide an innovative approach, studying endometriosis under the genital and gut microbiota scope. To realize this project, the investigators are planning to dose cfDNA to assess the oxidative stress caused by endometriosis and study its epigenetics. At the same time, the investigators will take a pragmatic approach by assessing pesticide exposure in these patients and estimate the correlation between gut or genital dysbiosis and chemical agent exposure. Also, the investigators will take the initiative to use classic culture, qPCR techniques, and NGS to establish signatures in vaginal, endometrial and gut microbiota in patients with endometriosis. With these approaches, the goal is to gain more knowledge about endometriosis and optimize early diagnosis by establishing a signature in the genital and gut microbiota, but also by dosing the cfDNA. By doing so the investigators could open new opportunities to develop new therapeutic strategies for endometriosis.

NCT Number	NCT07471373
Lead Sponsor	Centre Hospitalier Universitaire, Amiens
Conditions	Endometriosis, Infertility, Female Fertility, Cell Free DNA, Genital Microbiota, Vaginal Microbiota, Endometrial Microbiota, Pesticides +2 more
Enrollment	160 participants
Start Date	2026-02-27
Primary Completion	2028-02 (estimated)
Study Completion	2028-05 (estimated)
Updated on ClinicalTrials.gov	2026-05-15