Development of an Innovative Method to Extract Cord Blood Stem Cells With High Efficiency

The present study has the purpose to develop an innovative method for obtaining HSCs with a high yield. HSCs extracted from UCB using the traditional method followed by several steps of centrifugation will be compared to the new method which exploit a new biocompatible liquid, capable to separate the HSCs without causing mechanical stress to cells. The potential of ozone treatment to stimulate cells proliferation will be also assessed. Since ozone has been identified as an adjuvant to sustain cytoprotective pathways increasing cell performance, HSCs obtained from UCB samples will be treated by flowing ozone at low concentrations (between 2 and 20 μg/mL) in a mixture 1:1 with oxygen.

NCT Number	NCT07468201
Lead Sponsor	Fondazione IRCCS Policlinico San Matteo di Pavia
Conditions	HSCs Extraction
Enrollment	12 participants
Start Date	2024-04-10
Primary Completion	2026-04-10 (estimated)
Study Completion	2026-04-10 (estimated)
Updated on ClinicalTrials.gov	2026-03-12