Spatiotemporal Single-cell Atlas of Peri-implant Soft Tissue Healing

This study aims to explore the dynamic changes in different cell types and their molecular regulatory mechanisms during the healing process of peri-implant soft tissues, using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST). The research will focus specifically on the healing of soft tissues around dental implants in human subjects with the use of a healing abutment. Participants in the healing abutment group will have a micro healing abutment placed immediately after implant placement. Tissue samples will be collected at various time points (Day 4, Day 14, and Day 70) to construct a detailed single-cell map of the healing process. Spatial transcriptomics will be integrated to preserve tissue architecture, enabling the identification of the spatial distribution of different cell types and their interactions within the peri-implant microenvironment. This combined approach will allow for a comprehensive characterization of cellular interactions, spatial gene expression patterns, and regulatory networks specific to healing abutments. The goal is to identify key regulatory factors that could improve peri-implant soft tissue healing and help prevent peri-implantitis.

NCT Number	NCT07279090
Lead Sponsor	Stomatological Hospital Affiliated with Fujian Medical University
Conditions	Peri-implant Soft Tissue Healing
Enrollment	5 participants
Start Date	2025-12-20
Primary Completion	2026-12-01 (estimated)
Study Completion	2026-12-31 (estimated)
Updated on ClinicalTrials.gov	2025-12-16