Effect of Subtle Energy Transmission and Tao Calligraphy Mindfulness Practice on Mitochondrial DNA in Peripheral Blood Leukocytes

The goal of this pilot clinical trial is to learn if a subtle energy transmission and Tao Calligraphy Mindfulness Practice works to improve mitochondrial DNA. The main questions it aims to answer are: * Does Tao Calligraphy Mindfulness practice improve mitochondrial DNA content in peripheral blood leucocytes in adults? * Will this improvement of mitochondrial DNA content in peripheral blood leucocytes be statistically significant? Researchers will compare the values of mitochondrial DNA content in peripheral blood leucocytes at beginning of the mindfulness practices to the values at 3 months, 6 months and 12 months of practice group. Participants will: * have blood samples will drawn at accredited institutions and analyzed for Mitochondrial DNA content in Leucocytes upon entry into the study - Baseline time point; at 3-months time point, at six-months time point and at twelve-months time point. * All participants will complete the set of 3 questionnaires upon entry into the study - Baseline time point; at 3-months time point, at six-months time point and at twelve-months time point. * Practice the Mindfulness with Tao Calligraphy minimum 30 minutes daily.

NCT Number	NCT07261111
Lead Sponsor	Sha Research Foundation
Conditions	Mitochondrial DNA, Quality of Life
Enrollment	50 participants
Start Date	2026-07-02
Primary Completion	2030-12-31 (estimated)
Study Completion	2031-12-31 (estimated)
Updated on ClinicalTrials.gov	2026-07-14