Quantification of Progenitor Cell Circulation Following Vascular Resistance Exercise Using Delfi Tourniquet System

The purpose of this observational study is to examine how slowing down blood flow to the legs- a practice called blood flow restriction (BFR)- during exercise by applying compression to the legs affects the way stem cells are released into the blood stream. This will be determined by drawing 6 cubic centimeters (ccs) of blood immediately post-exercise after the Delfi Personalized Tourniquet System (PTS) has been removed. 6 ccs of blood will also be taken prior to exercise and at the 20-, 40-, and 60-minute marks after exercise. The main question of this study is: • Will the levels of stem cells extracted before and after exercise be the same if blood flow is restricted during exercise? In this study, participants will undergo the following: * 6 ccs of blood extracted from the forearm prior to exercise to establish a baseline * 3 exercises- seated leg extension, semi-reclined leg press, and seated hamstring curl- consisting of 4 sets of 30-15-15-15 repetitions per exercise; resistance will be set to 30% of one-rep maximum (1-RM) * Additional 6 cc blood draws will be performed immediately post-exercise and after 20, 40, and 60 minutes have elapsed

NCT Number	NCT07213726
Lead Sponsor	Andrews Research & Education Foundation Collaborators: Florida
Conditions	Blood Flow Restriction (BFR) Training Effects
Enrollment	15 participants
Start Date	2022-08-16
Primary Completion	2023-11-20 (estimated)
Study Completion	2026-11-20 (estimated)
Updated on ClinicalTrials.gov	2025-12-08