Antibacterial Efficacy and Outcomes of Root Canal Irrigation Methods

This randomized clinical trial aims to evaluate the antibacterial efficacy and treatment outcomes of three different final irrigation protocols during root canal therapy: 2.5% sodium hypochlorite alone, sodium hypochlorite with Passive Ultrasonic Irrigation (PUI), and sodium hypochlorite with XP-Endo Finisher activation. Sixty-six single-rooted teeth with pulp necrosis and apical periodontitis will be randomly assigned to one of the three groups. Antibacterial efficacy will be assessed through Real-Time PCR quantification of total bacterial load and four specific bacterial species before and after treatment. Treatment success will be evaluated clinically and radiographically at 6 and 12 months. The study aims to identify the most effective irrigation strategy for bacterial reduction and healing outcomes.

NCT Number	NCT06959602
Lead Sponsor	National and Kapodistrian University of Athens
Conditions	Pulp Necrosis, Apical Periodontitis, Endodontic Treatment
Enrollment	66 participants
Start Date	2025-04-01
Primary Completion	2025-09-01 (estimated)
Study Completion	2025-10-31 (estimated)
Updated on ClinicalTrials.gov	2025-06-03