Milk Consumption and Blood Microbiome in Healthy Volunteers

The goal of this study is to learn if cfDNA of milk-associated intestinal bacteria (MAIB) is detectable in the bloodstream. The primary question it aims to answer is: \- Does milk consumption simultaneously increase the number of MAIB cfDNA counts in blood samples, as it does in fecal samples? Researchers will compare cfDNA counts of MAIB to those of fecal samples to assess whether the blood microbiome is a valid measure of the gut microbiome, utilizing a self-developed bioinformatics pipeline. Participants will: * Abstain from consuming any dairy products for ten days, followed by drinking milk for 7 consecutive days. * Provide blood and fecal samples before dairy abstinence, as well as at 0, 24, and 48 hours, and one week after the reintroduction of milk.

NCT Number	NCT06944002
Lead Sponsor	Erasmus Medical Center Collaborators: NWO (Dutch Organisation for Scientific Research)
Conditions	Healthy
Enrollment	10 participants
Start Date	2025-07-10
Primary Completion	2025-09-15 (estimated)
Study Completion	2025-09-15 (estimated)
Updated on ClinicalTrials.gov	2025-08-07