Exhaled Breath Condensate Analysis in Mechanically Ventilated Patients

Mechanically ventilated intensive care patients will be sampled for a small amount of exhaled breath condensate from the ventilator circuit and for venous blood. Proteomic analysis of the exhaled breath condensate will be performed using mass spectrometry and in the blood sample, corresponding changes in the DNA, RNA, proteins, and metabolites will be studied. Resulting profiles will be correlated with routinely monitored parameters in order to identify patterns corresponding to various pathologies in order to enable their early detection.

NCT Number	NCT06189924
Lead Sponsor	The Institute of Molecular and Translational Medicine, Czech Republic
Conditions	Acute Respiratory Failure, Critical Illness
Enrollment	200 participants
Start Date	2026-03-01
Primary Completion	2028-12-31 (estimated)
Study Completion	2029-12-31 (estimated)
Updated on ClinicalTrials.gov	2026-06-25